Tag: Rf

Curve Digitalizer Publish tab showing the tlc_amino_acids_features Dataset and the selected accepted calibration.

GX-TXT Curve Digitalizer: From a TLC Photograph to Retention Factors with Python

Written by Giux | Published on Nov 14, 2026 | No comments

How can a thin-layer chromatography (TLC) photograph become quantitative scientific data? In this tutorial, we use GX-TXT Curve Digitalizer to calibrate a real TLC plate, digitize five annotated spots, and identify the baseline and solvent front. A reusable Python script calculates retention factors (Rf), publishes the results as a scientific Dataset, and enables interactive plotting and analysis.

#Chromatographic Analysis #Curve Digitalizer #GX-TXT #Image Analysis #Python #Retention Factor #Rf #Scientific Datasets #scientific digitization #Script Bridge #Thin-Layer Chromatography #TLC